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T lymphocytes are characterized by the expression of a repertoire of gamma and delta receptors. However, the extent of the T-cell receptor (TCR) usage in human thymocytes is unclear. To define TCR repertoire of human thymocytes, we analyzed the TCR alpha-chain repertoire of CD1-CD4-CD8- thymocytes in the light of the analysis of peripheral T-cell populations. The TCR V alpha and V beta genes were cloned from 13 thymocyte cDNA libraries obtained from pediatric thymuses, from clonal cultures of fetal thymocytes, and from thymuses, peripheral blood, and T-cell clones of patients with monogenic and/or immunodeficiency disorders. DNA sequencing showed that in thymocytes, the human V alpha 4 and V alpha 6 subfamilies were preferentially used, and we observed rare usage of human V beta 11 and V beta 12 subfamilies. On the contrary, no preferential usage of V alpha and V beta subfamilies was found in the peripheral T-cell compartment. In clonal T-cell populations, we found that both the V alpha 4 and V alpha 6 were in germline configurations and that the same V beta 11 or V beta 12 junctions were used. However, in five thymocyte libraries, we found that different V alpha and V beta junctions were used. These results indicate that the TCR repertoire in human thymocytes is not random, but restricted to some V alpha and V beta subfamilies.package scanner
import (
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type cmdList []string
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